Molecular detection and isolation of infectious laryngotracheitis virus (ILTV) in layer farms of Waset province, Iraq

Infectious Laryngotracheitis (ILT) is an acute highly contagious respiratory disease of chickens. It has significant economic importance due to mortalities and the decrease in egg production. In this study, twenty nine samples from different layer farms were collected outbreaks that occurred waset province, Iraq, at period November 2021 till March 2022 detect ILTV by molecular test through real time polymerase chain reaction assay (RT-PCR) as well isolation on embryonated chicken eggs chorioallantoic membrane (CAM) route. The clinical signs examination infected birds revealed extend head with expectoration bloody mucous, gasping weeping eye (watery eye). Gross pathological lesions catarrhal, caseated, fibrinonecrotic, hemorrhagic tracheitis. RT-PCR amplification specific for glycoprotein G gene ILT virus. Preparation Inoculation tissue which giving positive results (ECE) appeared white pock inoculated CAM second passage.